| Grdzelishvili, Valery, Ph.D. |
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Assistant Professor Molecular Virology, virus-host interactions, exploiting viruses as anticancer (“oncolytic”) agents
Academic Degrees
Professional Experience
Grdzelishvili's Lab 2010 (left to right): 
To Prospective Graduate Students! Thank you for your interest in our Molecular Virology lab. If you are interested in pursuing a graduate degree in my lab, I encourage you to contact me directly by email (). I am looking for exceptional, hard-working students to join our lab group!
Research Interests The major objective of my laboratory is to understand the mechanisms of viral RNA replication and transcription in the nonsegmented negative-strand RNA viruses (the order Mononegavirales). This order includes many important human and animal pathogens, such as rabies, measles, Ebola, Marburg, Nipah, Hendra, and other viruses. Importantly, the Ebola and Marburg viruses are classified as category A bioweapon agents and are currently considered to be one of the bioterrorism threats. A detailed understanding of the mode RNA synthesis in these viruses is fundamental to developing reagents to prevent and combat these deadly diseases. Another direction of my research is focused on the exploitation of negative-strand RNA viruses (vesicular stomatitis virus in particular) as clinically important anticancer (“oncolytic”) agents.
Vesicular stomatitis virus (VSV) life cycle 
VSV
Ebola virus
Sendai virus
Courses Taught
Important Links
Selected Publications Murphy A. M., Besmer D. M., Moerdyk-Schauwecker M., Moestl N., Ornelles D., Mukherjee P., and Grdzelishvili V. Z. 2012 “Vesicular stomatitis virus as an oncolytic agent against pancreatic ductal adenocarcinoma” Journal of Virology, published ahead of print 11 January 2012 , doi:10.1128/JVI.05640-11 Moerdyk-Schauwecker M., Destephanis D., Hastie E, Grdzelishvili V.Z. 2011 “Detecting Protein-Protein Interactions in Vesicular Stomatitis Virus Using a Cytoplasmic Yeast Two Hybrid System. Journal of Virological Methods. 173(2), 203-12. [PMID: 21320532]. Murphy A.M., Moerdyk-Schauwecker M., Mushegian A., Grdzelishvili V.Z. 2010 “Sequence-Function Analysis of the Sendai virus L protein domain VI” Virology, Jul 5. 405(2), 370-82. [PMID: 20609457] Shah N., Sunderland A., Grdzelishvili V.Z. 2010 "Cell Type Mediated Resistance of Vesicular Stomatitis Virus and Sendai Virus to Ribavirin", PLoS One, 5(6):e11265. [PMID: 20582319] Furr S.R., Moerdyk-Schauwecker M., Grdzelishvili V.Z., and Marriott I. 2010 "RIG-I Mediates Nonsegmented Negative-Sense RNA Virus-Induced Inflammatory Immune Responses of Primary Human Astrocytes" Glia, Jun 23. [Epub ahead of print], [PMID: 20578054] Chauhan, V.S., Sterka, D.G. Jr., Furr, S.R., Marriott, I. and Grdzelishvili, V.Z. 2010 “Vesicular stomatitis virus infects resident cells of the central nervous system and induces replication-dependent inflammatory responses” Virology, 400:187-96. Moerdyk-Schauwecker M., Hwang S. and Grdzelishvili V.Z. 2009 "Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach" Virology Journal, 6:166. Murphy A.M. and Grdzelishvili V.Z. 2009 "Identification of Sendai Virus L Protein Amino Acid Residues Affecting Viral mRNA Cap Methylation" Journal of Virology, 83(4):1669-81. Furr S.R., Chauhan V.S., Sterka D.Jr., Grdzelishvili V., and Marriott I. 2008 “Characterization of retinoic acid-inducible gene-I expression in primary murine glia following exposure to vesicular stomatitis virus”, Journal of Neurovirology, 14(6): 503 - 513. Grdzelishvili V.Z., Smallwood S., Tower D., Hall R.L., Hunt D.M., and Moyer S.A. 2006 “Identification of a new region in the vesicular stomatitis virus L protein which determines defects in mRNA cap methylation”, Virology 350, 394-405 Grdzelishvili V.Z., Smallwood S., Tower D., Hall R.L., Hunt D.M., and Moyer S.A. 2005 “A single amino acid change in the L polymerase protein of vesicular stomatitis virus completely abolishes viral mRNA cap methylation”, Journal of Virology 79, 7327-7337 Grdzelishvili V.Z., Garcia-Ruiz H., Watanabe T., and Ahlquist P. 2005 “Mutual interference between genomic RNA replication and subgenomic RNA transcription in brome mosaic virus”, Journal of Virology 79, 1438-1451 Kushner D.B, Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., and Ahlquist P. 2003 “Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus”, Proc. Natl. Acad. Sci. USA 100, 15764-15769 Grdzelishvili V.Z., Chapman S.N., Dawson W.O. and Lewandowski D.J. 2000 “Mapping of the tobacco mosaic virus movement protein and coat protein subgenomic RNA promoters in vivo”, Virology 275, 177-192 Solovyev, A. G., Savenkov E. I., Grdzelishvili V. Z., Kalinina N. O., Morozov S. Y., Schiemann J., and Atabekov J. G. 1999 “Movement of hordeivirus hybrids with exchanges in the triple gene block”, Virology 253, 278-287 Solovyev, A. G., Zelenina D. A., Savenkov E. I., Grdzelishvili V. Z., Morozov S. Y., Maiss E., Casper R., and Atabekov J. G.. 1997 “Host-controlled cell-to-cell movement of a hybrid barley stripe mosaic virus expressing a dianthovirus movement protein”, Intervirology 40,1-6 Solovyev, A. G., Zelenina D. A., Savenkov E. I., Grdzelishvili V. Z., Morozov S. Y., Lesemann D. E., Maiss E., Casper R., and Atabekov J. G.. 1996 “Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein”, Virology 217, 435-441. Selected Meeting Papers: Murphy A. and Grdzelishvili V.Z., 2011 “Vesicular stomatitis virus as an oncolytic agent against pancreatic ductal adenocarcinoma”,30th Annual Meeting of the American Society for Virology, July 16-20, Minneapolis, Minnesota, Abstracts, W15-12. Furr S. R., Grdzelishvili V.Z., and Marriott I. 2011 “The cytosolic sensors RIG-I and DAI can mediate glial inflammatory responses to neurotropic viruses” The 98th Annual Meeting of the American Association of Immunologists, May 13-17, San Francisco, Abstracts, 162.17 Murphy A., Dahlia Besmer, Pinku Mukherjee, and *Grdzelishvili V.Z., 2011 “Vesicular stomatitis virus as an oncolytic agent against pancreatic ductal adenocarcinoma”, The 6th International Conference on Oncolytic Viruses as Cancer Therapeutics, March 16-19, Las Vegas, Nevada, Abstracts, P28 Furr S., Moerdyk-Schauwecker M., Grdzelishvili V.Z., and Marriott I. 2010 “RIG-I mediates rhabdovirus-induced inflammatory immune responses of primary human astrocytes” The 97th Annual Meeting of the American Association of Immunologists, May 7-11, Baltimore. J. Immunol. 2010, 184, 136.3 *Grdzelishvili V.Z. and Moerdyk-Schauwecker M. 2010 “Identification and characterization of cellular proteins incorporated within virions of vesicular stomatitis virus and Sendai virus”, 29th Annual Meeting of the American Society for Virology, Bozeman, Montana July 17-21, Abstracts, W13-5. Shah N., Sunderland A., and Grdzelishvili V.Z., 2009 “Cell type dependent antiviral effect of ribavirin on replication of two distinct negative-strand nonsegmented RNA viruses”, 28th Annual Meeting of the Ameri can Society for Virology , Vancouver, BC, Canada, July 10-15, Abstracts Murphy A. and Grdzelishvili V.Z., 2009 “Functional analysis of the methyltransferase domain of the Sendai virus large polymerase protein”, 28th Annual Meeting of the American Society for Virology, Vancouver, BC, Canada, July 10-15, Abstracts, P2-7. Moerdyk-Schauwecker M. and Grdzelishvili V.Z., 2009 “Analysis of virion incorporated host proteins in two Mononegavirales using mass spectrometry”, 28th Annual Meeting of the American Society for Virology, Vancouver, BC, Canada, July 10-15, Abstracts, W5-7. Marriott, I, Chauhan, V.S., Sterka, D.G, and Grdzelishvili, V.Z. 2009. “Vesicular stomatitis virus infects primary murine microglia and astocytes and induces replication-dependent inflammatory cytokine production” The 96th Annual Meeting of the American Association of Immunologists, May 8-12, Seattle WA. J. Immunol. 182:133.47. Grdzelishvili V.Z. and Murphy A. 2008 “Critical amino acid residues for mRNA cap methylation in the large polymerase protein of Sendai virus”, XIIVth International Congress of Virology, Istanbul, Turkey, August 10-15, Abstracts, VOP-219 Murphy A. and Grdzelishvili V.Z., 2008 “Identification of the residues within the Sendai virus large polymerase protein essential for mRNA cap methylation”, 27th Annual Meeting of the American Society for Virology, Ithaca, NY, Cornell University July 12-16, Abstracts, W45-9 Grdzelishvili V. Z., S. Smallwood, D. Tower, R.L. Hall, D.M. Hunt, and S.A. Moyer. 2005 “Single amino acid changes in the L protein of vesicular stomatitis virus confer host restriction and defects in viral mRNA cap methylation” XIIIth International Congress of Virology, San Francisco, CA, July 23-28, Abstracts, 234V Grdzelishvili V. Z., S. Smallwood, D. Tower, R.L. Hall, D.M. Hunt, and S.A. Moyer. 2005 “Single amino acid changes in the L protein of vesicular stomatitis virus confer host restriction and defects in viral mRNA cap methylation” 24th Annual Meeting of the American Society for Virology, State College, Pennsylvania, June 18-22, Abstracts Grdzelishvili V. Z. and P. Ahlquist. 2003 “Interference between subgenomic RNA transcription and genomic RNA3 accumulation in brome mosaic virus” 22nd Annual Meeting of the American Society for Virology, Davis, California, July 12-16, Abstracts, number W8-6 Kushner, D.B., Lindenbach, B.D., Grdzelishvili, V.Z., Paul, S.M., and Ahlquist, P. 2003. “Systematic functional genomics cataloging of host gene functions in brome mosaic virus replication”. 22nd Annual Meeting of the American Society for Virology, Davis, California, July 12-16, Abstracts, number W33-1 Grdzelishvili V. Z. and P. Ahlquist 2002 “High-throughput screening of BMV mutants to separate replication and subgenomic RNA transcription functions” 21st Annual Meeting of the American Society for Virology, Lexington, Kentucky, July, 20-24, Abstracts, number W47-2 Knapp E., Grdzelishvili V. Z., G.M. Danyluk and D.J. Lewandowsky 2002 “ The role of trancated 126-kDa protein in movement of TMV defective RNAs” 21st Annual Meeting of the American Society for Virology, Lexington, Kentucky, July, 20-24, Abstracts, number W20-8. Kim K., P. Angeletti, Grdzelishvili V. Z., P. Ahlquist and P. Lambert. 2001 “Stable replication of human papillomavirus 16 in Saccharomyces cerevisiae” ICRF/UCSF 2001 DNA Tumour Virus Meeting, Churchill College, Cambridge, England, July, 24-29, number 6. Grdzelishvili V. Z., D.J. Lewandowski and W.O. Dawson. 2000 “Mapping of the subgenomic mRNA promoter for the movement protein gene of tobacco mosaic virus in vivo“ 19th Annual Meeting of the American Society for Virology, Fort Collins, Colorado, July, 8-12, Abstracts, number W27-8 |
